purification of LpxA of Chlamydia trachomatis and preparation of its polyclonal antibody.  


i-dna

The expression and purification of LpxA of Chlamydia trachomatis and preparation of its polyclonal antibody.

 

The aim of this research is to purify the LpxA protein of Chlamydia trachomatis (Ct) and put together the polyclonal antibody towards LpxA protein, in order to put a basis for learning the perform of LpxA protein. The LpxA gene was amplified by PCR. The expression plasmid pET28a-LpxA was constructed by utilizing pET28a because the vector.

 

The fusion protein containing 6 histidine tag was induced by IPTG and purified by Ni2+ chromatography gel. The purified His-LpxA protein was used as an immunogen to immunize New Zealand rabbits subcutaneously via the again to arrange polyclonal antibody. Immunoblotting was used to detect the response between the antibody and His-LpxA.

The willpower of polyclonal antibody titer was detected by ELISA. The relative molecular weight of His-LpxA was 32.eight kDa, and it may very well be expressed in Escherichia coli. The purity of the purified protein was about 95%. After immunizing New Zealand rabbits, the antiserum was in a position to acknowledge the recombinant His-LpxA protein with a titer larger than 1:10240. On this research, LpxA protein was efficiently purified and antiserum was ready, which supplied an experimental foundation for learning the perform of LpxA protein.

 

i-dna
i-dna

Anti-MFN2 antibody

STJ114644 100 µl
EUR 277
Description: This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

Anti-Mfn2 antibody

STJ94105 200 µl
EUR 197
Description: Mfn2 is a protein encoded by the MFN2 gene which is approximately 86,4 kDa. Mfn2 is localised to the mitochondrion outer membrane. It is involved in pink/parkin mediated mitophagy, toll-like receptor signalling pathways and glucose / energy metabolism. It is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. Mfn2 is ubiquitously expressed at low levels. Mutations in the MFN2 gene may result in Charcot-Marie-Tooth disease. STJ94105 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Mfn2 protein.

anti- MFN2 antibody

FNab05151 100µg
EUR 505.25
Description: Antibody raised against MFN2

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-MFN2 Antibody (Biotin)

STJ501760 100 µg
EUR 586

Anti-MFN2 Antibody (FITC)

STJ501761 100 µg
EUR 586

MFN2 Antibody

ABD8106 100 ug
EUR 438

MFN2 antibody

70R-18496 50 ul
EUR 435
Description: Rabbit polyclonal MFN2 antibody

MFN2 antibody

70R-13734 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal MFN2 antibody

MFN2 Antibody

DF8106 200ul
EUR 304
Description: MFN2 Antibody detects endogenous levels of total MFN2.

MFN2 Antibody

1-CSB-PA003234
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000

MFN2 Antibody

1-CSB-PA013756GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

MFN2 Antibody

1-CSB-PA013756LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:200-1:3000, IHC:1:20-1:200

MFN2 Antibody

33015-100ul 100ul
EUR 252

Anti-Mitofusin 2/MFN2 Antibody

PA1051 100ug/vial
EUR 294

Anti-Mitofusin 2/MFN2 Antibody

PB9265 100ug/vial
EUR 294

Mfn2 Polyclonal Antibody

ABP51785-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of Mfn2 from Human, Mouse, Rat. This Mfn2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Mfn2

Mfn2 Polyclonal Antibody

ABP51785-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of Mfn2 from Human, Mouse, Rat. This Mfn2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Mfn2

Mfn2 Polyclonal Antibody

ABP51785-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of Mfn2 from Human, Mouse, Rat. This Mfn2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Mfn2

Polyclonal MFN2 Antibody

APR08441G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MFN2 . This antibody is tested and proven to work in the following applications:

Mfn2 Polyclonal Antibody

ES2784-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Mfn2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Mfn2 Polyclonal Antibody

ES2784-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Mfn2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Mfn2 Polyclonal Antibody

41141-100ul 100ul
EUR 252

Mfn2 Polyclonal Antibody

41141-50ul 50ul
EUR 187

MFN2 siRNA

20-abx924043
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

MFN2 siRNA

20-abx924044
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

MFN2 Antibody, HRP conjugated

1-CSB-PA013756LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

MFN2 Antibody, FITC conjugated

1-CSB-PA013756LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

MFN2 Antibody, Biotin conjugated

1-CSB-PA013756LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MFN2. Recognizes MFN2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mitofusin-2 (MFN2) Antibody

20-abx121952
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Mitofusin 2 (MFN2) Antibody

20-abx113830
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Mitofusin 2 (MFN2) Antibody

abx445068-100ug 100 ug
EUR 523

Mitofusin-2 (MFN2) Antibody

abx235151-100ug 100 ug
EUR 481

Mitofusin-2 (MFN2) Antibody

20-abx301797
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mitofusin 2 (MFN2) Antibody

20-abx328857
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Mitofusin 2 (MFN2) Antibody

abx448536-100ug 100 ug
EUR 523

Mitofusin-2 (MFN2) Antibody

abx034129-400ul 400 ul
EUR 523

Mitofusin-2 (MFN2) Antibody

abx034129-80l 80 µl
EUR 286

Mitofusin-2 (MFN2) Antibody

20-abx136081
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Polyclonal MFN2 Antibody (Center)

APR08442G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MFN2 (Center). This antibody is tested and proven to work in the following applications:

Mfn2 Polyclonal Conjugated Antibody

C41141 100ul
EUR 397

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

MFN2 Blocking Peptide

DF8106-BP 1mg
EUR 195

MFN2 cloning plasmid

CSB-CL013756HU-10ug 10ug
EUR 747
Description: A cloning plasmid for the MFN2 gene.

pCMV-SPORT6-MFN2

PVT13137 2 ug
EUR 391

MFN2 Blocking Peptide

20-abx161675
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Mitofusion 2/MFN2

RA26001 100 ul
EUR 539

Mitofusin-2 (MFN2) Antibody (HRP)

20-abx314874
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mitofusin-2 (MFN2) Antibody (FITC)

20-abx314875
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mitofusin-2 (MFN2) Antibody (Biotin)

20-abx314876
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mitofusin 2 (MFN2) Antibody (ALP)

abx442465-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (APC)

abx442746-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (Biotin)

abx443026-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (FITC)

abx443306-100ug 100 ug
EUR 565

Mitofusin 2 (MFN2) Antibody (HRP)

abx443587-100ug 100 ug
EUR 565

Mitofusin 2 (MFN2) Antibody (PerCP)

abx444149-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (RPE)

abx444430-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (Streptavidin)

abx444711-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ALP)

abx446781-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (APC)

abx446782-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (PerCP)

abx446787-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (RPE)

abx446788-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (Streptavidin)

abx446789-100ug 100 ug
EUR 578

MFN2 ELISA KIT|Human

EF007293 96 Tests
EUR 689

pCMV-SPORT6-MFN2 Plasmid

PVTB00467 2 ug
EUR 356

pCMV-HA-MFN2 Plasmid

PVTB00467-2a 2 ug
EUR 356

MFN2 Recombinant Protein (Human)

RP019252 100 ug Ask for price

MFN2 Recombinant Protein (Mouse)

RP150413 100 ug Ask for price

MFN2 Recombinant Protein (Rat)

RP211526 100 ug Ask for price

Mouse MFN2 shRNA Plasmid

20-abx980316
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human MFN2 shRNA Plasmid

20-abx956672
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mitofusin 2 (MFN2) Antibody (ATTO 390)

abx440217-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 488)

abx440498-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 565)

abx440779-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 594)

abx441060-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 633)

abx441341-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 655)

abx441622-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 680)

abx441903-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 700)

abx442184-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 390)

abx446773-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 488)

abx446774-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 565)

abx446775-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 594)

abx446776-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 633)

abx446777-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 655)

abx446778-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 680)

abx446779-100ug 100 ug
EUR 578

Mitofusin 2 (MFN2) Antibody (ATTO 700)

abx446780-100ug 100 ug
EUR 578

Mfn2 ORF Vector (Rat) (pORF)

ORF070510 1.0 ug DNA
EUR 506

MFN2 ORF Vector (Human) (pORF)

ORF006418 1.0 ug DNA
EUR 95

Mfn2 ORF Vector (Mouse) (pORF)

ORF050139 1.0 ug DNA
EUR 506

MFN2 ELISA Kit (Human) (OKCD00471)

OKCD00471 96 Wells
EUR 831
Description: Description of target: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.5 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.2"Dysregulation of HSG triggers vascular proliferative disorders."_x005F_x005F_x000D_Chen K.-H., Guo X., Ma D., Guo Y., Li Q., Yang D., Li P., Qiu X., Wen S., Xiao R.-P., Tang J._x005F_x005F_x000D_Nat. Cell Biol. 6:872-883(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISEASE.Ref.9"Control of mitochondrial morphology by a human mitofusin."_x005F_x005F_x000D_Santel A., Fuller M.T._x005F_x005F_x000D_J. Cell Sci. 114:867-874(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-109; 622-GLY--VAL-624 AND 657-LYS--ARG-659.Ref.10"Membrane topology and mitochondrial targeting of mitofusins, ubiquitous mammalian homologs of the transmembrane GTPase Fzo."_x005F_x005F_x000D_Rojo M., Legros F., Chateau D., Lombes A._x005F_x005F_x000D_J. Cell Sci. 115:1663-1674(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-109; SER-110 AND ARG-259.Ref.16"MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission."_x005F_x005F_x000D_Palmer C.S., Elgass K.D., Parton R.G., Osellame L.D., Stojanovski D., Ryan M.T._x005F_x005F_x000D_J. Biol. Chem. 288:27584-27593(2013) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.17"PINK1-phosphorylated mitofusin 2 is a Parkin receptor for culling damaged mitochondria."_x005F_x005F_x000D_Chen Y., Dorn G.W. II_x005F_x005F_x000D_Science 340:471-475(2013) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN MITOPHAGY, INTERACTION WITH PARK2, PHOSPHORYLATION AT THR-111 AND SER-442 BY PINK1, UBIQUITINATION BY PARK2, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-111 AND SER-442. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.055 ng/mL

MFN2 ELISA Kit (Human) (OKEH08248)

OKEH08248 96 Wells
EUR 896
Description: Description of target: This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 35pg/mL

Mitofusin 2 (MFN2) Antibody (PE/ATTO 594)

abx443868-100ug 100 ug
EUR 592

Mitofusin 2 (MFN2) Antibody (PE/ATTO 594)

abx446786-100ug 100 ug
EUR 592

 

Efficiency analysis of the polyclonal anti-rabies virus ribonucleoprotein IgG antibodies produced in-house to be used in direct fluorescent antibody check.

 

Fluorescein isothiocyanate (FITC) labelled anti-rabies virus ribonucleoprotein (RNP) antibodies can be utilized as immunoreagents in direct fluorescent antibody testing (dFAT) for rabies diagnoses. Whereas in-house merchandise are sometimes utilized by laboratories, most conjugates are business reagents. Business anti-RNP antibodies are solely accessible for analysis functions in Brazil, nevertheless, which contributes to the rising use of in-house produced antibodies.

 

Contemplating that conjugate high quality might affect the outcomes obtained throughout rabies analysis, we sought to investigate the efficiency necessities of in-house produced polyclonal anti-RNP IgG-FITC for utility in dFAT. To that finish, their reproducibility, diagnostic sensitivity, and specificity have been evaluated. The titer of polyclonal anti-RNP IgG-FITC was initially decided and evaluated by dFAT, utilizing central nervous system (CNS) samples of various animal species (canines, cats, bovines, equines, bats, and non-human primates).

 

As our predominant end result, the polyclonal anti-RNP IgG-FITC reached a titer of 1:30/1:40 in dFAT, with 100% of diagnostic sensitivity and specificity. When it comes to reproducibility, the antibodies, regardless the manufacturing batch, introduced the identical performances. In conclusion, the in-house produced polyclonal anti-RNP IgG-FITC proved appropriate for rabies virus antigen detection by dFAT.

 

Manufacturing of F(ab’)2 from Monoclonal and Polyclonal Antibodies.

 

Antibodies are broadly utilized in therapeutic, diagnostic, and analysis purposes, and antibody derivatives reminiscent of F(ab’)2 fragments are used when solely a specific antibody area is required. F(ab’)2 will be produced via antibody engineering, however some purposes require F(ab’)2 produced from an authentic formulated antibody or instantly from a polyclonal antibody pool.

The cysteine protease immunoglobulin-degrading enzyme (IdeS) from Streptococcus pyogenes digests immunoglobulin G (IgG) particularly and effectively to supply F(ab’)2 . Right here we element the manufacturing and purification of recombinant IdeS; its utilization to digest monoclonal or polyclonal antibodies to F(ab’)2 fragments; and F(ab’)2 purification via consecutive affinity chromatography steps.

The resultant F(ab’)2 exhibit excessive purity, retain antigen-binding performance, and are readily utilizable in numerous downstream purposes. © 2020 by John Wiley & Sons, Inc. Primary Protocol: Manufacturing and purification of F(ab’)2 fragments from monoclonal and polyclonal antibodies utilizing IdeS Alternate Protocol: Purification of polyclonal antigen-specific F(ab’)2 fragments from human serum or secretions Help Protocol: Manufacturing and purification of IdeS.

 

Analysis of liquid and powdered types of polyclonal antibody preparation towards Streptococcus bovis and Fusobacterium necrophorum in cattle tailored or not tailored to extremely fermentable carbohydrate diets.

 

Feed components that modify rumen fermentation can be utilized to forestall metabolic disturbances reminiscent of acidosis and optimize beef cattle manufacturing. The research evaluated the consequences of liquid and powdered types of polyclonal antibody preparation (PAP) towards Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that have been tailored or unadapted to a excessive focus weight-reduction plan.

A double 3 × Three Latin sq. design was used with three PAP therapies (management, powdered and liquid PAP) and two adaptation protocols (tailored, unadapted; utilized to the sq.). Tailored animals have been transitioned for two weeks from an all-forage to an 80% focus weight-reduction plan, whereas unadapted animals have been switched abruptly.

 

Interactions between sampling time and adaptation have been noticed; 12 h after feeding, the tailored group had decrease ruminal pH and larger complete brief chain fatty acid concentrations than the unadapted group, whereas the alternative was noticed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen focus have been typically larger in tailored than unadapted cattle as much as 36 h after feeding.

 

Adaptation promoted 3.5 instances the variety of Entodinium protozoa however copy numbers of S. bovis and Fibrobacter succinogens genes in rumen fluid weren’t affected. Nonetheless, neither liquid nor powdered types of PAP altered rumen acidosis variables in tailored or unadapted animals.Adaptation of cattle to extremely fermentable carbohydrate diets promoted a extra secure ruminal setting, however PAP was not efficient on this research by which no animal skilled acute or sub-acute rumen acidosis.

Anti-DNMT3A antibody

STJ23413 100 µl
EUR 277
Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

anti- DNMT3A antibody

FNab02484 100µg
EUR 505.25
Description: Antibody raised against DNMT3A

anti- DNMT3A antibody

FNab02485 100µg
EUR 505.25
Description: Antibody raised against DNMT3A

Anti-DNMT3A antibody

STJ119211 100 µl
EUR 277

Anti-DNMT3A antibody

STJ113373 100 µl
EUR 277
Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

anti-Dnmt3a

YF-PA11403 50 ug
EUR 363
Description: Mouse polyclonal to Dnmt3a

anti-Dnmt3a

YF-PA11404 100 ul
EUR 403
Description: Rabbit polyclonal to Dnmt3a

anti-Dnmt3a

YF-PA11405 100 ug
EUR 403
Description: Rabbit polyclonal to Dnmt3a

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

DLR-DNMT3A-Hu-48T 48T
EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human DNA Methyltransferase 3A (DNMT3A) in samples from tissue homogenates, cell lysates or other biological fluids.

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

DLR-DNMT3A-Hu-96T 96T
EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human DNA Methyltransferase 3A (DNMT3A) in samples from tissue homogenates, cell lysates or other biological fluids.

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

RD-DNMT3A-Hu-48Tests 48 Tests
EUR 521

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

RD-DNMT3A-Hu-96Tests 96 Tests
EUR 723

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

RDR-DNMT3A-Hu-48Tests 48 Tests
EUR 544

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

RDR-DNMT3A-Hu-96Tests 96 Tests
EUR 756

Anti-Dnmt3a Rabbit Monoclonal Antibody

M00136 100ug/vial
EUR 397
Description: Rabbit Monoclonal Dnmt3a Antibody. Validated in IF, WB and tested in Human, Rat.

Anti-DiMethyl-DNMT3A-K44 antibody

STJ118467 100 µl
EUR 393

DNMT3A Antibody

ABD6795 100 ug
EUR 438

DNMT3A Antibody

ABD7226 100 ug
EUR 438

DNMT3A antibody

70R-16903 50 ul
EUR 435
Description: Rabbit polyclonal DNMT3A antibody

DNMT3a Antibody

3227-100
EUR 349

DNMT3a Antibody

3227-30T
EUR 146

DNMT3A Antibody

DF6795 200ul
EUR 304
Description: DNMT3A Antibody detects endogenous levels of total DNMT3A.

DNMT3A Antibody

DF7226 200ul
EUR 304
Description: DNMT3A Antibody detects endogenous levels of total DNMT3A.

DNMT3A Antibody

1-CSB-PA045919
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:100-1:300

DNMT3A Antibody

1-CSB-PA632606
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DNMT3A Antibody

1-CSB-PA094007
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:100-1:300

DNMT3A Antibody

1-CSB-PA522891
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DNMT3A Antibody

1-CSB-PA007083GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC

DNMT3A Antibody

1-CSB-PA007083LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

DNMT3A antibody

38611-100ul 100ul
EUR 252

DNMT3A antibody

38966-100ul 100ul
EUR 252

DNMT3A Antibody

32580-100ul 100ul
EUR 252

Dnmt3a Antibody

48869-100ul 100ul
EUR 333

Dnmt3a Antibody

48869-50ul 50ul
EUR 239

Anti-Dnmt3a (mouse) Monoclonal Antibody (64B1446)

M00136-1 100ug
EUR 446
Description: Mouse Monoclonal Dnmt3a (mouse) Antibody (64B1446). Validated in IP, IF, IHC, WB and tested in Mouse.

Dnmt3a/ Rat Dnmt3a ELISA Kit

ELI-09442r 96 Tests
EUR 886

DNMT3A polyclonal antibody

6849-25
EUR 370

DNMT3A Polyclonal Antibody

A-1003 100 µl
EUR 616.95
Description: kits suitable for this type of research

DNMT3A Polyclonal Antibody

A52569 100 µg
EUR 570.55
Description: kits suitable for this type of research

Dnmt3a Conjugated Antibody

C48869 100ul
EUR 397

DNMT3A Conjugated Antibody

C32580 100ul
EUR 397

pEGFP- DNMT3A

PVT10312 2 ug
EUR 266

DNMT3A Protein

E11000 10 µg
EUR 809.8
Description: fast delivery possible

DNMT3A siRNA

20-abx914453
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DNMT3A siRNA

20-abx914454
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DNMT3A siRNA

20-abx901552
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC941898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF594 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC941898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF594 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCA1898-250 250uL
EUR 394
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), APC conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC811898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF680R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC811898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF680R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCAP1898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCAP1898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCB1898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Biotin conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCB1898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Biotin conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC471898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF647 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC471898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF647 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC551898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF555 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC551898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF555 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC431898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF543 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC431898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF543 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC881898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF488A conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC881898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF488A conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCH1898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCH1898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCP1898-250 250uL
EUR 394
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), PerCP conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC041898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF405S conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC041898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF405S conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNCR1898-250 250uL
EUR 394
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), RPE conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNUB1898-100 100uL
EUR 264
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Concentration: 0.2mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNUB1898-50 50uL
EUR 405
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), 1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNUB1898-500 500uL
EUR 513
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), Concentration: 0.2mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC611898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF660R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC611898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF660R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC681898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF568 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC681898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF568 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC701898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF770 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC701898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF770 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC051898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF405M conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC051898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF405M conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC401898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF640R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC401898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF640R conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC801898-100 100uL
EUR 233
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF680 conjugate, Concentration: 0.1mg/mL

DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2) Antibody

BNC801898-500 500uL
EUR 545
Description: Primary antibody against DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF680 conjugate, Concentration: 0.1mg/mL

DNMT3A Antibody, HRP conjugated

1-CSB-PA007083LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

DNMT3A Antibody, FITC conjugated

1-CSB-PA007083LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DNMT3A. Recognizes DNMT3A from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

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